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Abstract July 2009

Abstract 2009
+ Abstract July 2009

 

ABSTRACTS IJBB VOLUME 6 NO. 3. JULY 2009


MICROVESICULA OTOLITHI N.GEN., N.SP. (TREMATODA: HEMIURIDAE: LECITHASTIRINAE) FROM THE FISH OTOLITHUS ARGENTEUS (C.V.) OF KARACHI COAST, PAKISTAN

Fatima Mujib Bilqees1, Bushra Khalil1, Aly Khan2 and M. Farooq Haseeb3

1Department of Zoology, Jinnah University for Women, Karachi-74600, Pakistan
2Crop Diseases Research Institute, PARC, University of Karachi, Karachi-75270, Pakistan
3Department of Zoology, University of Karachi, Karachi-75270, Pakistan

ABSTRACT

A new hemiurid trematode Microvesicula otolithi n.gen., n.sp. is described here from the fish Otolithus argenteus of Karachi coast. This trematode is characterized by having a more or less spindle-shaped body without tail, oral sucker small, pharynx and esophagus are also small, ceca long and thick reaching to near about the posterior end, acetabulum 3 or more times larger than oral sucker, testes almost symmetrical posterior to this, seminal vesicle small, pear-shaped, genital opening posteroventral to pharynx. Ovary is bean-shaped, larger and posterior to testes, seminal receptacle larger than ovary and postovarian. Vitelline tubules 7 radiating from posterior to ovary and laterally extending to the cecal and extracecal regions, situated in posterior third of the body. Uterus extending from preovarian to prebifurcal region, with numerous, small eggs.  

 

TWO NEW SPECIES OF THE GENUS SCOTTIOLA UVAROV (ORTHOPTERA: GRYLLIDAE: NEMOBIINAE) FROM PAKISTAN

Nasreen Khan and Syed Kamaluddin       

Department of Zoology, Federal Urdu University of Arts, Science and technology, Gulshan-E-Iqbal Campus, University Road Karachi-75400, Pakistan.

ABSTRACT

Two new species of the genus Scottiola Uvarov are described to accommodating one already described species in detail with reference to their genital components. A key to the Pakistani species is formulated and the cladistic relationship of included texa is also discussed by using their apomorphies.

 

QADRIANA OTOLITHI N.SP., (HEMURIDAE: DINURINAE LOOSS, 1907) FROM THE FISH OTOLITHUS ARGENTEUS (SCIAENIDAE) OF KARACHI COAST, PAKISTAN

Fatima Mujib Bilqees1, Rana Hadi1, Khatoon, N2, Muti-ur-Rahman2 and Shakila Perveen1

 1Department of Zoology, Jinnah University for Women, Karachi, Pakistan

2Department of Zoology, University of Karachi, Karachi, Pakistan

ABSTRACT

A new hemiurid trematode is described here parasitizing the intestine of the fish Otolithus argenteus of Karachi coast. Qadriana otolithi n.sp., is the third species of the genus Qadriana Bilqees, 1971. It is compared with previous species and is regarded new due to morphometric variations in the diagnostic features. Main differences are in the position of seminal vesicle, genital opening, ovary and vitellaria.

 

PROSORCHIS MACROACETABULUM N.SP. (TREMATODA: HEMIURIDAE) FROM THE FISH STROMATEUS NIGER (BL.) OF KARACHI COAST, PAKISTAN

Fatima Mujib Bilqees1, Bushra Khalil1, M. Farooq Haseeb2 and Muti-Ur-Rehman3

1Department of Zoology, Jinnah University for Women, Karachi-74600, Pakistan
2Department of Zoology, Shipowners College, Karachi, Pakistan
3Department of Zoology, University of Karachi, Karachi-75270, Pakistan

ABSTRACT

A new hemiurid trematode Prosorchis macroacetabulum is described here from the fish Stromateus niger (Bl) collected from the fish harbour, Karachi coast, Pakistan. Body of this fluke is dorsoventrally flattened, wide. Oral sucker is subterminal, prepharynx absent, pharynx comparatively small, esophagus indistinct, ceca wide projecting anteriorly towards oral sucker and reaching to the posterior end of the body. Acetabulum more than three times larger than oral sucker, almost equatorial. Testes two, preacetabular, seminal vesicle anterior to testes, slightly twisted, pars prostatica small, well differentiated, hermaphroditic duct is also small, genital pore ventral to posterior border of pharynx. Ovary is postacetabular and postequatorial, receptaculum seminis small, vitellaria composed of seven, long, convoluted tubules, uterus reaching to near about posterior region of the body, joining hermaphroditic duct anteriorly at the level of cecal bifurcation, eggs numerous, small, excretory vesicle is Y-shaped.

 

ETHNOBOTANICAL STUDIES OF VASCULAR BIODIVERSITY IN JANDOOL VALLEY DISTRICT DIR (L)

Asad Ullah1, Abdur Rashid2 and Siraj-Ud-Din3

1Centre of Plant Biodiversity, University of Peshawar, Pakistan

2Department of Botany University of Peshawar, Pakistan 3Department of Botany, Islamia College Peshawar, Pakistan

ABSTRACT

One hundred and thirty two species were collected from the research area. These species were spread into 65 families, among them 61 families were angiosperms, 54 of them were dicot and 7 were monocot, while 3 families were pteridophytes and one was gymnosperm. Poaceae was the leading one with 13 species, followed by Solanaceae with 7 species and Polygonaceae with 6 species. All these species were divided in to two categories viz. medicinally important plants and species of multiple uses. A total of sixty (60) plants belonging to 43 families were medicinally important, among them 40 were angiosperms, in which 37 were dicot and 3 were monocot, while 2 were pteridophytes and one was gymnosperm. One hundred and four (104) species belonging to 55 families were of multiple uses, in which 52 were angiosperms, among them 47 were dicot and 5 were monocot, 2 were pteridophytes and one was gymnosperm (Table no. 1).

 

GENOTYPES OF HELICOBACTER PYLORI IN SYMPTOMATIC PATIENTS OF KARACHI, PAKISTAN

Muhammad Hanif1, Ajaz Rasool2, Parveen Zaidi1, Abid Hameed1 and Laeeque Ahmed3

1PCR and Molecular Biology lab, Karachi Institute of Radiotherapy and Nuclear Medicine,
2 Laboratory of Molecular Genetics, Department of Microbiology, University of Karachi,
 3Department of Gastroenterology, Liaquat National Hospital, Karachi.

Abstract

Helicobacter pylori infections and gastroduodenal diseases have been reported several times in this region but there is no data regarding prevalence of H. pylori VacA and CagA genotypes in this part of the world. The objective of this study was to determine the prevalence of H. pylori genotypes in different gastroduodenal disease patients of Karachi.

Multiple biopsy samples were taken from 227 patients of peptic ulcer disease (PUD), chronic gastritis (CG) and/or abdominal discomfort (AD) in the gastroenterology departments from three different health institutions of Karachi and processed for rapid urease test, culture and histopathology. Positive isolates were further confirmed by PCR amplification of the phosphoglucosamine mutase gene (glmM) and analyzed for the occurrence of CagA and VacA genotypes by PCR. A significant association between H. pylori infection and gastroduodenal diseases was observed (p<0.001). Out of 227 biopsy samples 120 (52.8%) were confirmed positive for H. pylori in which 56 were that of PUD, 37 with CG, 19 with AD and 8 with gastric cancer (GCa). Overall 92% isolates from patients with PUD, CG, AD and GCa were CagA positive. Eighty five (70.8 %) of 120 strains had VacA signal sequence genotype s1a, 19 (16%) had type s1b and 16 (13%) had type s2. The VacA middle region type m1 and m2 were detected in 78(65%) and 42 (35%) strains. The combinations s1a/m1 and s1a/m2 were found in 66(55%) and 19 (15.8%) respectively. The s1b/m1 and s1b/m2 were found in 12(10%) and 8(6.6%) strains respectively. The s2m2 combinations were identified in 15(12.5%) of 120 isolates. The s2m1 combination was not found in any strain. Forty eight (85.7%) of 56 patients with PUD, 25 (67.5%) of 37 patients with CG, 5 (26.3%) of 19 patients with AD and 7 (87.5%) of 8 with GCa harbored type s1a strains.  Genotypes s1a/m1 with positive CagA are commonly associated with PUD, CG, AD and GCa.

 

VARIABILITY AND HERITABILITY UNDER NORMAL AND RAINFED CONDITIONS IN ASTIVUM WHEAT (TRITICUM ASTIVUM L.)

Kalimullah1, Rahm Din1, Muhammad Jamil Khan2, Said Ghulam2  and Ibrarullah3

1Department of PBG, Faculty of Agri:, Gomal Univ: D.I.Khan. kalimpbgian@yahoo.com
2Department of Soil Science, Faculty of Agri:, Gomal Univ: D.I.Khan. shahmir3rd@yahoo.com
3 Department of Horticulture, Faculty of Agri:, Gomal Univ: D.I.Khan. ibree106@hotmail.com

ABSTRACT

A set of 22 experimental wheat lines along with four check cultivars were evaluated in irrigated and unirrigated environments with objectives to determine genetic and phenotypic variation and heritability estimates for yield and its traits. The two environments were statistically at par for grains spike-1, spike weight, biological yield, and grain yield except days to heading. Highly significant genetic variability existed among wheat lines (P ≤ 0.01) in the combined analysis across two test environments for traits except spike weight. Genotypes x environment interactions were non-significant for traits indicating consistent performance of lines in two test environments. However lines and check cultivars were two to five days early in heading under unirrigated environment compared to the irrigated. Spike weight and grains spike-1 also reduced under unirrigated environments. Genetic variances were greater than environmental variances for most of traits.  Heritability estimates were of higher magnitude (0.74 to 0.96) for days to heading, spike length, biological yield and grain yield, and low for spike weight in both the environments. The differential heritability for yield and yield related traits suggests simultaneous evaluation of wheat lines under the two environments.
 

STUDY ON THE DETERMINATION OF SUITABLE COUNT OF A BLENDED YARN WITH THE MAXIMUM UTILIZATION OF JUTE IN THE BLEND

M. Abul Kalam Azad1, Nazmina Chowdhury1, Shamina Jafrin1 and M. Tahzibul Haque (Shahin)2

1Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207, Bangladesh
2Khulna University of Engineering & Technology (KUET)

ABSTRACT

Blending is an important factor for producing a particular count of quality yarn. A study on the jute cotton blended yarn of different ratios viz.,  jute: cotton /40:60, jute: cotton / 50: 50, jute: cotton / 60:40, jute: cotton / 70: 30 and jute: cotton/ 80: 20 was performed. Of different counts of yarn ( 20s , 15s  & 10s ), it was observed that the 10s yarn of jute : cotton/ 50 : 50 ratio gave better results than that of the other two types of yarns of the same blend ratios.

 

COMPARATIVE STUDY ON THE PROPERTIES OF DENIM FABRICS PRODUCED WITH BLENDED ROTOR SPUN YARN AND 100% COTTON YARN

M. Abul Kalam Azad1, M. Kamal Uddin1, Shamina Jafrin1 and M. Tahzibul Haque (Shahin) 2

1Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207, Bangladesh. and
2Khulna University of Engineering & Technology (KUET), sjafrin@gmail.com

ABSTRACT

An assessment on the properties of blended (50: 50 / jute: cotton) denim fabric has been made in terms of different treatments. The results have been compared with those of the properties of 100% cotton denim fabric. It is observed that the properties of blended denim fabric is very similar and comparable to that of the 100% cotton denim fabric at the end use stage that is at the final treatment stage.

 

DETERMINATION OF FATTY ACID COMPOSITIONAL FINGERPRINTS OF DIFFERENT VEGETABLE OILS AND THEIR EFFECTIVENESS FOR ADULTERATION DETECTION IN COMMERCIAL OILS

Zafar Iqbal

Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box-128, Jhang Road, Faisalabad, Pakistan

ABSTRACT

Authenticity of vegetable oils is of concern for commercial and health reasons. Fatty acid composition in the seed oil of locally grown crops like corn, canola, soybean, sunflower and cotton were determined by GC/FID. From these fatty acids compositional fingerprints certain factors were derived to be used as purity criteria for specific vegetable oil. The derived factors were applied for the detection of adulteration in market samples of different edible oils (corn, canola, soybean and sunflower). The results revealed that corn, canola and soybean oil of different brands were pure, but all the sunflower oil samples were found adulterated at varying levels (low to high). One brand, labeled sunflower oil, contained 100% canola oil.

 

BIOLOGICAL AND CHEMICAL EFFECT OF SOIL CONDITIONER- LIGNITE HUMIC ACID ON SUNFLOWER (HELIANTHUS ANNUUS L.) PLANT GROWTH AND FATTY ACID CONSTITUENTS

Saqib Nasir1, Aisha Nelofar2, M. Ashraf1 and Tahira B. Sarfaraz 3
1Fuel Research Centre, PCSIR Karachi-75280, Pakistan
2 Pharmaceutical Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan 
3H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

Abstract

Field trials of sunflower (Helianthus annuus L.) were conducted with different concentrations of Pakistani lignite derived humic acid. The present study included seed treatment with 0.050, 0.075, 0.125 and 0.200 % aqueous ammonium humate along with control crop for data evaluation. Furthermore, oil content of seeds obtained from treated and untreated plant seeds using n-hexane and their physicochemical properties were compared with standard values. The fatty acid analysis of sunflower seeds oil was performed by gas chromatography after successive extractions. Encouraging results were obtained for seed oil content, unsaturated hydrocarbons and plant yield. The physicochemical properties (proximate analysis) of oil did not show any significant change in the oil composition. The oils extracted from seeds obtained from treated plants showed relatively higher content (47.2 %) of linoleic acid (C18:2).

 

COMPARATIVE EFFICIENCY OF BIO-POWER AND N CHEMICAL FERTILIZER IN SUPPORT OF GROWTH, YIELD AND N UPTAKE OF WHEAT

*Muhammad Ali1, Muhammad Saleem2 and Syed Haider Abbas2

1Horticulture Research Institute, NARC, Islamabad
2*Crop Sciences Institute, National Agricultural Research Centre, Islamabad

ABSTRACT

 A field research was undertaken to evaluate the comparative efficiency of Bio-power (a multi microbe) and nitrogen (N) chemical fertilizer; alone and in combination on the growth, grain yield and N uptake of wheat. The chemical fertilizer was band incorporated after sowing the crop. The experiment was laid out under Randomized Complete Block Design (RCBD) having 4 replications. The seeds were treated with bio-fertilizer before sowing. Significant effect was recorded only on 1000 grain weight but all the treatments performed at par except control. The treatment 100 kg N ha-1 resulted in 30.21 % increase in 1000 grain weight over control. Bio-power alone or along with ½ recommended N @ 50 kg ha-1 or full recommended dose of N fertilizer and N chemical fertilizer did not significantly affect grain yield, biomass and N uptake. Insignificant difference was established between bio-power and N chemical fertilizer to have any effect on the growth, grain yield and N uptake in wheat. However, the maximum increase in grain yield and N uptake was attained with 100 kg N ha-1.

 

EFFECTS OF PHOTOSYNTHETIC PIGMENTS OF VIGNA RADIATA L. &VIGNA UNGUICULATA L. CULTIVATED IN LEAD CONTAMINATED SOIL AMENDEDWITH CHICKEN MANURE AND MINERAL FERTILIZER

Neelofer Hamid and Faiza Jawaid

Department of Botany, University of Karachi, Karachi-75270, Pakistan

ABSTRACT

Vigna radiata and Vigna unguiculata plants were grown in lead (Pb) contaminated soil which is amended with different concentrations of organic fertilizer (chicken manure) and mineral fertilizer (NPK). In present investigation polluted soil was from industrial area of Karachi with elevated Pb content. The concentration of photosynthetic pigments that is chlorophyll “a”, “b” and carotenoides in leaf of developing plants were monitored every one week interval up to the period of 4 weeks. Application of both fertilizer improve the concentration of  chlorophyll “a”, “b” and carotenoids, with time. In control of both Vigna radiata and Vigna unguiculata, chlorophyll “a”, “b” and carotenoids concentration was reduces, such redution in the concentration of leaf pigment due to the toxic effect of metals (Pb).

 

EFFECT OF CANNABIS SATIVA FORTIFIED FEED ON MUSCLE GROWTH AND VISCERAL ORGANS IN BROILER CHICKS

Rifat Ullah Khan1*, Fazil-e-Raziq Durrani2, Niela Chand2, Haseeb Anwar1, Shabana Naz3, Farooq Ahmad Farooqi4 and Muhammad Nauman Manzoor5

1Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan
2Department of Poultry Science, NWFP Agricultural University Peshawar, Pakistan
3Department of Zoology and Fisheries, University of Agriculture, Faisalabad, Pakistan
4Drug Residue Laboratories, Animal Quarantine Department, Karachi, Pakistan
5Department of Animal Nutrition, University of Agriculture, Faisalabad, Pakistan
*Corresponding author: email: rifatullahkhhan@gmail.com; Phone # +0092-03329089294

ABSTRACT

This paper describes the effect of Cannabis sativa seeds on growth performance of muscle and abdominal organs of broiler chicks. Total 160 broiler chicks (one day old) of equal weight were purchased from the local market and were randomly divided into four groups A, B, C and D. Each group having 40 chicks were further distributed into four replicates. Dried crushed seeds of Cannabis sativa seeds were added to the feed of group B, C and D at the rate of 5, 10, and 20% of offered feed respectively while group A served as a control. Feed and water were offered ad libitum. The studied parameters included weight of breast, thigh, leg, liver, gizzard, intestines, heart and abdominal fat. The experiment lasted for 42 days. Breast and leg weight were significantly higher (P < 0.05) in group D. It was also revealed from the data that weight of liver, gizzard, intestines and abdominal fat were significantly higher (P < 0.05) in group D, however, heart weight was non significant. It was concluded from these results that Cannabis sativa seeds have remarkable impact on weight of muscles and visceral organs in broilers which is always desirable in poultry industry.

 

ELIMINATION KINETICS OF CEFACLOR IN MALE HUMAN BEINGS
                             
Shafaq Ashfaq and Rakhshanda Nawaz,
 
Department of Chemistry. University of Agriculture, Faisalabad, Pakistan; e-mail: shafaq_ashfaq@yahoo.com 
                                             
ABSTRACT
        
Elimination kinetics of cefaclor was investigated in twelve male healthy volunteers after 375 mg single dose oral administration of the drug. Elimination kinetics parameters revealed that the mean value for rate constant (K10) was 0.30, mean value for elimination half-life was 2.25h, mean value for rate constant (K21) was 0.30, mean value for clearance was 4.04 1/h, mean value for mean residence time was 6.73h. No relation was found regarding age, weight, height and body temperature considering elimination kinetics

 

INDUCED PROMOSING EARLINESS VARIANTS FROM LATE MATURE COTTON CULTIVAR ASHOR 1 BY GAMMA RAYS IN IRAQ

M. A.AL-Hamdany and H.Y. Jaber

Agricultural Researches Directorate, Ministry of Science and Technology, P.O. Box. 765, Al-Jaderia Av.  Baghdad, Iraq

ABSTRACT

Host diversity in the very late maturity cotton cultivar Ashor 1 was created by gamma rays in order to select agronomical desirable earliness genetic variants. Seeds of cotton cultivar Ashor 1 were exposed to 250 Gy of gamma rays and  planted  with  untreated seeds. Selection for earliness was conducted in M2 progeny. Evaluation trails on the selected variants resulted in choosing ten promising variants. Earliness percentages of the selected variants ranged from 84.30 to 88.8 compared to 60.8% Ashor 1 cultivar. However, the productivity of these variants ranged from 85.0 to 133.30 g cotton flower/plant while it was 49.20 g in Ashor 1 in the first picking (160 days after seed sowing).  The selected variants also surpassed their origin in total yield of both first and second picking (one month later).  Fiber length was not affected in eight variants while remarkable reduction was observed in two variants.                  

 

RESISTANCE PATTERN OF GRAM NEGATIVE UROPATHOGENS ISOLATED FROM COMMUNITY ACQUIRED URINARY TRACT INFECTIONS AGAINST QUINOLONES

Sabahat Saeed1 and Perween Tariq2

1 Department of Microbiology, Jinnah University for Women, Karachi, Pakistan.
2 Department of Microbiology, University of Karachi, Karachi Pakistan.

ABSTRACT

The fluoroquinolones are synthetic compounds with greater potency and a broader antimicrobial activity than their quinolone precursor, nalidixic acid, which is used only as urinary antiseptic. Susceptibility tests of  345 strains, belonging to 9 species of Gram negative uropathogens against quinolones, were carried out by disc diffusion method. This comprised Escherichia coli (270 strains), Klebsiella pneumoniae (51), K. ozaenae (03), Proteus mirabilis (05), Pseudomonas aeruginosa (10), Salmonella typhi (01), S. paratyphi A (02), S. paratyphi B (01) and Serratia marcescens (02). The highest rate of resistance was recorded against ciprofloxacin and nalidixic acid i.e. each 6.1% (21/345) followed by ofloxacin (4.3%, 15/345) and norfloxacin (0.3%, 1/345). Continued surveillance is required to prevent the future spread of these resistancet strains.

 

STRUCTURE, COMPOSITION, PHYTOMASS AND NET PRIMARY PRODUCTIVITY IN A CENCHRUS SETIGERUS VAHL. DOMINATED COMMUNITY IN MOIST-SALINE HABITAT OF KARACHI, PAKISTAN

D. Khan

 

 

 

 
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